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13-Jul-2020 20:42

In practice, this unique capability is negated by the low throughput of the method.

Field-mediated membrane poration has supplanted chemical methods in many delivery applications, particularly those involving nongene target molecules and primary cells.

The cell membrane is a selectively permeable barrier between a cell and its environment, regulating passage of material into and out of the cell.

Membrane transport is fundamental to the intrinsic functioning of the cell with several natural mechanisms (e.g., passive diffusion, active and co-transport, and endocytosis/exocytosis) permitting cellular uptake and secretion of small and large molecules.

DNA transfection is inherently more challenging than nongene delivery.

Here we discuss the mechanism of this combined mode operation in detail, including in-depth characterization of capabilities for delivery of plasmid DNA.

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Efficiency of these methods is comparable to microinjection due to single-cell scale treatment; however, parallel arrays of flow constrictions in microchannels (2D) or orifice plates (3D) yield much higher throughput.

This is not true of larger macromolecules, which require alternative means to enter the cell interior.

Ideal delivery systems also protect materials from cytoplasmic degradation, convey materials to a target location, and facilitate action on that target.

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wikia JWPlayer=:wikia JWPlayer);define('wikia.article Video.featured Video.autoplay',['Test','wikia.article Video.featured Video.cookies',require.Direct delivery of nucleic acids as described in this work offers a compelling alternative that avoids the inherent shortcomings of viral vectors.



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